OK people! Sorry for being absent for such a long time, but life is just too busy to post new stuff.
So… Let’s see what I’ve been up to… Surviving grad school… so far… Got a paper accepted for print in RNA Biology. Working on another manuscript, making figures, etc. Rotating in a cool lab and probably will try to stay in it if I can.
But anyway, back to yeast. A couple months ago I tried contacting WYeast regarding my findings on their Berliner Blend (yes I know, I just can’t leave it alone!) because I’m pretty sure they used Pediococcus instead of Lactobacillus in their last blend. Here is what I got:
After banging my head against the wall trying to isolate the bugs in that blend I finally managed to get 3 types of colonies on the plates.
White, smooth, round colonies – probably Saccharomyces. Do not break down bromocresol green (resulting in blue-transparent colonies) nor universal indicator (in which case they are orange). No acid production. Under microscope they look like yeast. Round cells, about 5-6um in diameter. Budding visible.
Beige, smooth, round colonies. Break down bromocresol green and universal indicator resulting in white colonies. Low acid production. Under microscope they look like HUGE yeast cells. Round, tear-shaped, and rarely oblong, approx 11-17um in diameter/length. Pretty sure I saw some budding but didn’t take pictures. Seems to me like that’s the Brettanomyces spp. even though they don’t look like the traditional “brain shaped” colonies. B. annomalus perhaps? Who knows.
Small to gigantic, watery, transparent, smooth colonies. Do not metabolize indicators resulting in watery colonies on every medium. Acid production. Under microscope they look like cocci in chains. Approx 0.6um in diameter. Gram +. Looks like Pediococcus or Enterococcus to me, which is weird because WY says they have Lactobacillus in that blend. No matter how much I tried, I never got any bacillus colonies. Maybe they changed it in 2011 and used Pedio instead of Lacto.
Also I’m pretty sure I isolated the Brett from the Old Ale blend. I know it’s only Brett L which you can just buy, but this is more fun. Brain shaped colonies, oblong cells, nuclei visible with Meth. Blue stain.
Attached are some pictures. I apologize for the quality. My camera has something on the inside which results in showing all sorts of junk that’s not really there, not to mention poor quality.